Protein Labeling Reagents
Biotium CF570 Succinimidyl Ester
CF Dye Succinimidyl Esters (SE, also known as NHS esters) are amine-reactive fluorescent dyes. SE dyes are commonly used to label antibodies and other proteins on free amine groups, such as lysine residues. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Red fluorescent CF570 dye has excitation/emission at 568/591 nm.
Biotium CF647 Protein Labeling Kit , 3x(1mg) labelings
This kit contains everything you need to label antibodies or other proteins with a fluorescent CF dye succinimidyl ester (CF dye SE). Each kit contains reagents for three labeling reactions of up to 1 mg protein per reaction. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent CF647 dye has excitation/emission at 650/665 nm.
Biotium Biotin-XX Tyramide
CF dye tyramide conjugates can be used for tyramide signal amplification (TSA), which is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid in situ. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent CF640R dye has excitation/emission at 642/662 nm.Also see Biotium's CF Dye Tyramide Amplification Kits.
Biotium Fura-2, AM Ester, 20x50ug
Fura-2, AM ester is an intracellular fluorescent calcium indicator, and the preferred dye for ratiometric imaging microscopy. The membrane-permeant AM ester can be loaded into cells via incubation, but due to limited water solubility, a mild detergent Pluronic F-127 is often used to facilitate cell loading. Fura-2, AM ester itself does not bind Ca2, but intracellular esterases readily hydrolyze it to Fura-2. Upon calcium binding, the excitation maximum of Fura-2 undergoes a blue-shift from 363 nm (no Ca2) to 335 nm (high Ca2) while the emission maximum is relatively unchanged. Measure by flow cytometry, fluorescence microscopy, or certain fluoresence microplate readers. Properties: Light yellow solid soluble in DMSO; Store at -20°C, protect from light, especially in solution; Kd: 145 nM (22°C, pH 7.2 buffer); Ex/Em = 363/512 nm, e = 27,000 M-1cm-1 (no Ca2); Ex/Em 335/505 nm, e = 35,000 M-1cm-1 (high Ca2); C44H47N3O24; MW: 1001.9; [108964-32-5].
Biotium Phalloidin, CF594, 300 U
Phalloidin conjugates are actin filament stains for fixed and permeabilized cells. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Deep-red fluorescent CF594 has excitation/emission at 593/614 nm.
Biotium CF633 Hydrazide
Our CF dye hydrazides are bright, extremely water-soluble and nontoxic. These properties make the dyes excellent fixable polar tracers for microinjection into cells. Hydrazides also can be used for labeling aldehyde or ketone groups, however, for this application we recommend using the more reactive CF dye aminooxy forms. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent CF633 dye has excitation/emission at 630/650 nm.
Medchemexpress LLC HY-D0719 5g Medchemexpress, Fluorescein Diacetate CAS:596-09-8 Purity:>98%
Medchemexpress, HY-D0719 5g Fluorescein Diacetate CAS:596-09-8 Fluorescein diacetate is a cell permeable esterase-substrate. Fluorescein diacetate can be used as a fluorogenic substrate for hGSTP1-1. Purity:>98% Medchemexpress has over 10000 novel life-science reagents, reference compounds, APIs and natural compounds for laboratory and scientific use. Other quantity can also be offered.
MilliporeSigma TMB/E ULTRA SENSITIVE
TMB/E Ultra Sensitive, Blue, Horseradish Peroxidase Substrate (soluble)
Fluorochrome Inc Fluoro-Gold, 50 mg
Hydroxystilbamidine (also called Fluoro-Gold) has been used extensively as a retrograde tracer for neurons and also a histochemical stain. Fluoro-Gold can undergo retrograde axonal transport, it shows extensive filling of dendrites, and has a high resistance to fading.
Abcam 5(6)-Carbo x y- x -rhodamine, 25MG
MW 1069.2 Da, Purity >90%. Stable, long wavelength, water soluble fluorophore has found widespread use in DNA-labeling, cell staining and protein labeling applications.
The product is subject to the following: Abcam Restricted Use Statement